Influnence of siRNA interfering Annexina A1 expression on apoptosis of papillary thyroid carcinomar TPC-1 cells / 重庆医学

Objective To investigate the effect of ANX A1 on the biological characteristics of papillary thyroid carcinom cells by interfering with the expression of Annexina A1 (ANX A1) in papillary thyroid carcinoma cells through small interfering RNAs (siRNA).Methods The designed highly efficient siRNA was used to conduct the specific interfence on ANXA1 expression in the papillary thyroid carcinoma TPC-1 cells.The effect of ANXA1 on TPC-1 apoptosis in PTC was observed by flow cytometry.Results The designed siRAN could efficiently inhibit the expression of ANXA1 mRNA in PTC,enhanced the cell apoptosis in TPC-1 cells in vitro.Conclusion siRNA can interfere with the expression of ANXA1 and promote the apoptosis of papillary thyroid carcinoma which suggesting that ANXA1 may be an important biological target for the treatment of papillary thyroid carcinoma.

Health survey of painting and coating workers in an automobile manufacturing enterprise in Guangzhou, China / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the health status of painting and coating workers in an automobile manufacturing enterprise in Guangzhou, China and analyze the influential factors for the health status of these workers, and to provide health intervention measures and strategies.</p><p><b>METHODS</b>Typical sampling was used to select an automobile manufacturing enterprise; according to whether the subjects were in contact with paint and coatings, paint spraying workers in the painting workshop were selected as the exposed group, and the staff in the administration, procurement, and marketing departments as the control group. Physical examination was performed by doctors among these subjects.</p><p><b>RESULTS</b>The exposed group had significantly higher positive rates of dizziness, headache, bleeding gums, chest tightness, and skin itching than the control group (P < 0.05). The exposed group had a nonsignificantly higher rate of abnormal ECG than the control group (P > 0.05), but the exposed group had a significantly higher incidence of various blocks (P = 0.020) and significantly lower leukocyte count, erythrocyte count, and male workers' hemoglobin level (P < 0.05), as compared with the control group.</p><p><b>CONCLUSION</b>Volatile organic solvents have adverse effect on the health of paint spraying workers in the automobile manufacturing enterprise, so regular physical examination should be performed to strengthen health interventions and improve health.</p>